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Leukocyte cell adhesion molecules (LFA-1, Mac-1 and VLA-4) mobilization in circulating granulocytes were analysed by flow cytometry and endothelial cell adhesion molecules (ICAM-1 and VCAM-1) were detected by immunohistochemistry on lung sections.

studies were conducted on endothelial LT2 cells, stimulated with TNF-α, to evaluate ICAM-1, IL-8 and VEGF m RNA expression upon PACs treatment.

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There is thus an urgent need to identify new drug targets and develop new drugs against these pathogens. falciparum use multiple mechanisms to gain entry into their respective host cells.Here we report the identification of an 11-k Da synthetic protein, M5, that binds to human ICAM-1 and ICAM-4, as shown by in vitro interaction studies, surface plasmon resonance and immunolocalization.M5 greatly inhibits the invasion of macrophages and erythrocytes by Mycobacterium tuberculosis and Plasmodium falciparum, respectively. falciparum merozoites, and the addition of recombinant ICAM-4 to parasite cultures blocks invasion of erythrocytes by newly released merozoites.WHO (World Health Organization) studies indicate almost 8.6 million new cases and 1.3 million TB deaths in 2012 (ref. TB is caused by Mycobacterium tuberculosis (Mtb) that primarily, although not exclusively, invades lung phagocytic cells such as macrophages, neutrophils, monocytes and dendritic cells (DCs).The incidence of malaria is equally staggering, at 207 million cases, with 627,000 deaths reported by WHO for the year 2012 (ref. One of the main reasons for such high number of infections is the emergence of resistance to the currently available drugs, as well as a severe paucity of new drugs that can be added to the chemotherapy regimen.




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